Organ Model: Liver

Introduction

This protocol covers the general process for using the Liver-Chip R1 Co-Culture.

Introduction

This protocol covers the general process for using the Liver-Chip R1 Quad-Culture.

Introduction

This protocol describes the general steps for using the Emulate Chip-R1 Basic Research Kit.

Version 1.0 (Release Date: March 2022)

Introduction

These Compound Distribution Kit analysis tools simplify assay preparation and data analysis for the Emulate Compound Distribution Kit (CDK) protocol, enabling users to quantify the amount of test compound loss (e.g., absorption or adsorption) ahead of a Chip-S1^® Stretchable Chip experiment.

There are two Excel-based tools in the download: The Media Volume Preparation Calculator and the CDK Data Analysis Calculator. The Media Volume Preparation Calculator calculates the media volume required for the CDK assay, based on the intended flow rate, study duration, and expected re-dosing protocol. The CDK Data Analysis Calculator calculates the adjusted average cellular exposure and concentration range based on the dosing concentration.

Related Information:

• Protocol: Compound Distribution Kit Protocol
• Protocol: Compound Treatment Solution Preparation and Treatment

Version 1.0 (Release Date: November 2021)

Introduction

The Apparent Permeability (P_app) Calculator is an Excel-based tool that helps users calculate the permeability of the epithelial-endothelial barrier in Emulate Organ-Chips. The P_app of a molecule is determined by dosing the inlet of one channel, collecting the effluent of both channels, and calculating the amount of compound that crossed through the membrane over time. This tool can be used to help researchers streamline the process of assessing the barrier function of healthy or diseased tissues, as well as studying drug transport.

Related information:

• Product Pages: Colon Intestine-Chip, Duodenum Intestine-Chip, Proximal Tubule Kidney-Chip
• Protocols: Barrier Function Analysis
• Technical Note: Characterization of the Colon Intestine-Chip

Introduction

This document provides guidance for using the Pod Imaging Adapter, which organizes Pod Portable Modules and aligns them on the same viewing axis for quick cell viability and health checks.

Introduction

This is a revised version of the previously released “Basic Organ-Chip Culture” protocol (updated as of 22-Dec-2022)

This protocol covers the general process for using the Basic Research Kit.

Introduction

This protocol guides users through preparing AAV dosing media to use with Emulate Liver-Chips.

Version 1.0 (Release Date: October 2022)

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introduction

The Liver AAV Sample Dosing Calculator is an Excel-based tool that calculates adeno-associated virus (AAV) dilution in media, given the desired MOI, vector titer, and the number of chips.

Related Information: ​

• Product Page: Liver-Chip​
• Protocol: AAV Dosing and Solution Treatment Protocol​
• Endpoint Analysis Protocols: Morphology, Albumin, ALT​
• Application Note: Testing Transduction of AAV-Based Therapeutics on the Liver-Chip

Introduction

This method explains how to perform quantitative measurements of human alanine transaminase (ALT) protein from Emulate human Liver-Chip effluent samples.