The Colon Intestine-Chip provides a human organoid-based platform to study cell-cell interactions and mechanisms that elicit collapse of the intestinal epithelial homeostasis. We employed the cytokines IFN, a prototype cytokine on intestinal epithelial barrier disruption studies, or IL-22. IL-22 acts as a barrier-insulting cytokine when applied to healthy tissue in our Colon Intestine-Chip, in line with recent findings.
For barrier disruption of customer-originated cell material, this protocol may need to be optimized. Please reach out to Emulate Field Science Support for additional guidance.