Immunofluorescence Staining—Colon Intestine-Chip


This is the method we have developed for fixation and immunofluorescence staining for the Colon Intestine-Chip. We realize, however, that users may have their own fixation and staining processes that have been developed for specific cell types, antibodies, or antigens. If users would prefer to use other fixatives, permeabilizing solutions, or blocking buffers, they may do so while following the process outlined below.