The GSH-Glo™ Assay is a luminescent-based assay for the detection and quantification of glutathione (GSH), an antioxidant that can prevent damage to cellular components caused by reactive oxygen species such as free radicals, peroxides, and lipid peroxides. GSH is involved in the detoxification of both xenobiotic and endogenous compounds. A change in GSH levels can be used as an indicator of toxicity.
This protocol covers quantification of glycogen on our Organ-Chips.
Goal: Detect and measure secreted cytokines and chemokines from Emulate Organ-Chip effluent samples.
Goal: Quantify lactate dehydrogenase (LDH) levels from Emulate Organ-Chip effluent samples.
Keratin 18 (K18) is an intracellular protein expressed at high levels by many types of epithelial cells. During cell death, the cellular content of soluble K18 will be released into the extracellular compartment. The M65 EpiDeath® ELISA measures soluble keratin 18 (K18) (cytokeratin 18 [CK18]) released from dying cells and can be used in the research of overall cell death (due to apoptosis and necrosis) of epithelial cells.
Goal: Quantify hepcidin from Emulate Organ-Chip effluent.
Goal: Quantify cholesterol from Organ-Chip effluent.
Goal: Quantify albumin levels from Emualte human Organ-Chip effluent samples.
All fluorescent or confocal imaging of cells can be done directly in the Organ-Chip. You do not need to disassemble the chip, or isolate the membrane in order to image cells. The membrane is located 0.8 mm from the bottom of the chip and is visually accessible by most objectives on most microscopes, although the use of a long-working distance objective lens is recommended for optimal results. The chip is made of an optically transparent material that will not cause any significant distortion of your signal or image; it does not auto fluoresce.
