Fluorescence Imaging

Introduction

All fluorescent or confocal imaging of cells can be done directly in the Organ-Chip. You do not need to disassemble the chip, or isolate the membrane in order to image cells. The membrane is located 0.8 mm from the bottom of the chip and is visually accessible by most objectives on most microscopes, although the use of a long-working distance objective lens is recommended for optimal results. The chip is made of an optically transparent material that will not cause any significant distortion of your signal or image; it does not auto fluoresce.

Bright-Field & Phase-Contrast Imaging

Introduction

Goal: Perform bright-field and phase-contrast imaging of cells in Emulate Organ-Chips.

Live Staining of Reactive Oxygen Species Using CellROX®

Introduction

CellROX® is a fluorogenic probe for detecting cellular oxidative stress in live cells.

Live Staining of Mitochondria Using MitoTracker® Fluorescent Dyes

Introduction

MitoTracker® is a fluorescent dye that stains mitochondria in live cells. Its accumulation is dependent upon membrane potential. This endpoint is used as a marker of cell viability.

Live Staining of Lipid Droplets Using Nile Red

Introduction

Nile red is used to localize and quantify lipids, particularly neutral lipid droplets within cells.

Live Staining of Lipid Droplets Using AdipoRed

Introduction

AdipoRedTM is used to localize and quantify intracellular lipid droplets.

Live Staining of Functional Mitochondria Using TMRM

Introduction

How to perform live staining with tetramethylrhodamine, methyl ester (TMRM), a cell-permeant dye that accumulates in healthy mitochondria with intact membrane potentials.

Live Staining of Caspase-3/7

Introduction

CellEvent™ Caspase-3/7 Green Detection Reagent is a fluorogenic probe for detecting activated caspase-3/7 in apoptotic cells.

Live Staining of Cells

Introduction

Goal: Stain live cells in Emulate Organ-Chips.

Immunofluorescence Staining of Vimentin

Introduction

Goal: Visualize vimentin via fluorescent imaging in fixed Emulate Organ-Chips.