Introduction
Goal: Visualize expression of multidrug resistanceassociated protein (MRP-2) in fixed Emulate Organ-Chips.
Organ Model: Brain
Immunofluorescence Staining of Multidrug Resistance-Associated Protein (MRP-2)
Immunofluorescence Staining of F-actin
Introduction
Goal: Visualize F-actin via fluorescent imaging in Emulate Organ-Chips.
Immunofluorescence Staining of CD31
Introduction
CD31 or PECAM-1, is a type I integral membrane glycoprotein that is expressed at high levels on endothelial cells. CD31 staining can be used as a marker of endothelial cells. The expression on endothelial cells is concentrated at junctions between adjacent cells.
Immunofluorescence Staining of Alpha-Smooth Muscle Actin
Introduction
Goal: Visualize smooth-muscle actin filaments in fixed Emulate Organ-Chip.
Cell Lysis for RNA Isolation
Introduction
Goal: Lyse cells in Emulate Organ-Chips for RNA isolation.
Cell Lysis for Protein Extraction
Introduction
Goal: Cell lysis in Organ-Chips for protein extraction.
Effluent Volume Measurement
Introduction
Goal: Measure the effluent volume from Pod™ portable module reservoirs.
Effluent Sampling
Introduction
Goal: Sample effluent from Emulate Organ-Chips via Pod™ portable module reservoirs.
Compound Treatment Solution: Preparation and Treatment
Introduction
Goal: Prepare compound treatment solution and treat cells in Emulate Organ-Chips with test compounds.