Organ Model: Liver

Introduction

Goal: Visualize smooth-muscle actin filaments in fixed Emulate Organ-Chip.

Introduction

(As of August 14, 2023, a new version of the protocol has been uploaded.)

This protocol covers how to seed and culture a Quad-Culture Liver-Chip using the Chip-S1™ stretchable chip.

(As of August 14, 2023, a new version of the protocol has been uploaded.)

Introduction

This protocol covers how to seed and culture a Co-Culture Liver-Chip using the Chip-S1^® Stretchable Chip.

Introduction

Goal: Lyse cells in Emulate Organ-Chips for RNA isolation.

Introduction

Goal: Cell lysis in Organ-Chips for protein extraction.

Introduction

Goal: Measure the effluent volume from Pod™ portable module reservoirs.

Introduction

Goal: Sample effluent from Emulate Organ-Chips via Pod™ portable module reservoirs.

Introduction

Goal: Fixing and IF staining of cells in Emulate Organ-Chips.

Note: This is the method we have developed for fixation and immunofluorescence staining for our OrganChips. We realize, however, that users may have their own fixation and staining processes that have been developed for specific cell types, antibodies, or antigens. If users would prefer to use other fixatives, permeabilizing solutions, or blocking buffers, they may do so while following the process outlined below. This protocol has been optimized for fixation and staining of the Emulate Liver-Chip for our specific staining protocols.

Introduction

Goal: Prepare compound treatment solution and treat cells in Emulate Organ-Chips with test compounds.