Keratin 18 (K18) is an intracellular protein expressed at high levels by many types of epithelial cells. During cell death, the cellular content of soluble K18 will be released into the extracellular compartment. The M65 EpiDeath® ELISA measures soluble keratin 18 (K18) (cytokeratin 18 [CK18]) released from dying cells and can be used in the research of overall cell death (due to apoptosis and necrosis) of epithelial cells.
Goal: Quantify hepcidin from Emulate Organ-Chip effluent.
Goal: Quantify cholesterol from Organ-Chip effluent.
Goal: Quantify human aspartate transaminase (AST) from Liver-Chip effluent samples.
Goal: Quantify albumin levels from Emualte human Organ-Chip effluent samples.
All fluorescent or confocal imaging of cells can be done directly in the Organ-Chip. You do not need to disassemble the chip, or isolate the membrane in order to image cells. The membrane is located 0.8 mm from the bottom of the chip and is visually accessible by most objectives on most microscopes, although the use of a long-working distance objective lens is recommended for optimal results. The chip is made of an optically transparent material that will not cause any significant distortion of your signal or image; it does not auto fluoresce.
Goal: Perform bright-field and phase-contrast imaging of cells in Emulate Organ-Chips.
CellROX® is a fluorogenic probe for detecting cellular oxidative stress in live cells.
MitoTracker® is a fluorescent dye that stains mitochondria in live cells. Its accumulation is dependent upon membrane potential. This endpoint is used as a marker of cell viability.
Nile red is used to localize and quantify lipids, particularly neutral lipid droplets within cells.
