Introduction
Goal: Perform bright-field and phase-contrast imaging of cells in Emulate Organ-Chips.
Organ Model: Colon
Bright-Field & Phase-Contrast Imaging
Live Staining of Reactive Oxygen Species Using CellROX®
Introduction
CellROX® is a fluorogenic probe for detecting cellular oxidative stress in live cells.
Live Staining of Mitochondria Using MitoTracker® Fluorescent Dyes
Introduction
MitoTracker® is a fluorescent dye that stains mitochondria in live cells. Its accumulation is dependent upon membrane potential. This endpoint is used as a marker of cell viability.
Live Staining of Lipid Droplets Using Nile Red
Introduction
Nile red is used to localize and quantify lipids, particularly neutral lipid droplets within cells.
Live Staining of Lipid Droplets Using AdipoRed
Introduction
AdipoRedTM is used to localize and quantify intracellular lipid droplets.
Live Staining of Functional Mitochondria Using TMRM
Introduction
How to perform live staining with tetramethylrhodamine, methyl ester (TMRM), a cell-permeant dye that accumulates in healthy mitochondria with intact membrane potentials.
Live Staining of Caspase-3/7
Introduction
CellEvent™ Caspase-3/7 Green Detection Reagent is a fluorogenic probe for detecting activated caspase-3/7 in apoptotic cells.
Live Staining of Cells
Introduction
Goal: Stain live cells in Emulate Organ-Chips.
Immunofluorescence Staining of Vimentin
Introduction
Goal: Visualize vimentin via fluorescent imaging in fixed Emulate Organ-Chips.
Immunofluorescence Staining of Multidrug Resistance-Associated Protein (MRP-2)
Introduction
Goal: Visualize expression of multidrug resistanceassociated protein (MRP-2) in fixed Emulate Organ-Chips.