Introduction
AdipoRedTM is used to localize and quantify intracellular lipid droplets.
Organ Model: Colon
Live Staining of Lipid Droplets Using AdipoRed
Live Staining of Functional Mitochondria Using TMRM
Introduction
How to perform live staining with tetramethylrhodamine, methyl ester (TMRM), a cell-permeant dye that accumulates in healthy mitochondria with intact membrane potentials.
Live Staining of Caspase-3/7
Introduction
CellEvent™ Caspase-3/7 Green Detection Reagent is a fluorogenic probe for detecting activated caspase-3/7 in apoptotic cells.
Live Staining of Cells
Introduction
Goal: Stain live cells in Emulate Organ-Chips.
Immunofluorescence Staining of Vimentin
Introduction
Goal: Visualize vimentin via fluorescent imaging in fixed Emulate Organ-Chips.
Immunofluorescence Staining of Multidrug Resistance-Associated Protein (MRP-2)
Introduction
Goal: Visualize expression of multidrug resistanceassociated protein (MRP-2) in fixed Emulate Organ-Chips.
Immunofluorescence Staining of F-actin
Introduction
Goal: Visualize F-actin via fluorescent imaging in Emulate Organ-Chips.
Immunofluorescence Staining of Alpha-Smooth Muscle Actin
Introduction
Goal: Visualize smooth-muscle actin filaments in fixed Emulate Organ-Chip.
Cell Lysis for RNA Isolation
Introduction
Goal: Lyse cells in Emulate Organ-Chips for RNA isolation.
Cell Lysis for Protein Extraction
Introduction
Goal: Cell lysis in Organ-Chips for protein extraction.