Introduction
Goal: Visualize smooth-muscle actin filaments in fixed Emulate Organ-Chip.
Organ Model: Brain
Immunofluorescence Staining of Alpha-Smooth Muscle Actin
Cell Lysis for RNA Isolation
Introduction
Goal: Lyse cells in Emulate Organ-Chips for RNA isolation.
Cell Lysis for Protein Extraction
Introduction
Goal: Cell lysis in Organ-Chips for protein extraction.
Effluent Volume Measurement
Introduction
Goal: Measure the effluent volume from Pod™ portable module reservoirs.
Effluent Sampling
Introduction
Goal: Sample effluent from Emulate Organ-Chips via Pod™ portable module reservoirs.
Compound Treatment Solution: Preparation and Treatment
Introduction
Goal: Prepare compound treatment solution and treat cells in Emulate Organ-Chips with test compounds.