Introduction
Goal: Quantify lactate dehydrogenase (LDH) levels from Emulate Organ-Chip effluent samples.
Organ Model: Liver
LDH (Cytotoxicity) Assay
Hepcidin Quantification Assay
Introduction
Goal: Quantify hepcidin from Emulate Organ-Chip effluent.
Cholesterol Quantification Assay
Introduction
Goal: Quantify cholesterol from Organ-Chip effluent.
Aspartate Transaminase (AST) Assay
Introduction
Goal: Quantify human aspartate transaminase (AST) from Liver-Chip effluent samples.
Albumin Quantification Assay
Introduction
Goal: Quantify albumin levels from Emualte human Organ-Chip effluent samples.
Fluorescence Imaging
Introduction
All fluorescent or confocal imaging of cells can be done directly in the Organ-Chip. You do not need to disassemble the chip, or isolate the membrane in order to image cells. The membrane is located 0.8 mm from the bottom of the chip and is visually accessible by most objectives on most microscopes, although the use of a long-working distance objective lens is recommended for optimal results. The chip is made of an optically transparent material that will not cause any significant distortion of your signal or image; it does not auto fluoresce.
Bright-Field & Phase-Contrast Imaging
Introduction
Goal: Perform bright-field and phase-contrast imaging of cells in Emulate Organ-Chips.
Live Staining of Reactive Oxygen Species Using CellROX®
Introduction
CellROX® is a fluorogenic probe for detecting cellular oxidative stress in live cells.
Live Staining of Mitochondria Using MitoTracker® Fluorescent Dyes
Introduction
MitoTracker® is a fluorescent dye that stains mitochondria in live cells. Its accumulation is dependent upon membrane potential. This endpoint is used as a marker of cell viability.
Live Staining of Lipid Droplets Using Nile Red
Introduction
Nile red is used to localize and quantify lipids, particularly neutral lipid droplets within cells.