Quantification of Cytokines and Acute Inflammatory Phase Proteins Secretion

Introduction

This protocol uses the Colon Intestine-Chip as a reference point aiming to the quantitation of the secretion levels of cytokines (IL-1β, IL-6, IL-8, TNFα) and acute inflammatory phase proteins (SAA, CRP, VCAM-1, ICAM-1) under baseline conditions and upon basolateral stimulation with the pro-inflammatory cytokine interferon gamma (IFNγ). These methods and assay conditions could change with a different Organ-Chip.