Assessment of Cleaved Caspase-3


This protocol uses the Colon Intestine-Chip as a reference point aiming to the semi-quantitative assessment of the cleaved Caspase-3 levels under baseline conditions and upon basolateral stimulation with pro-inflammatory cytokines. These methods and assay conditions could change with a different Organ-Chip. Please reach out to Emulate Field Science Support for additional guidance.