Utilizing the Colon Intestine-Chip to Investigate Mechanisms of Gastrointestinal Disease

Organoids provide long-term viability, spatial organization, and cellular diversity representative of human intestinal epithelium. However, they don’t accurately mimic the architecture and the molecular signature of the intestinal epithelium. This limitation has been an impediment to allowing them to be harnessed for investigating mechanisms that drive leaky-gut syndrome in humans.

Emulate combines advancements in organoid and Organs-on-a-Chip technologies to develop a microphysiological Organ-on-Chip model designed to mimic properties of human intestinal epithelium to enable insights into barrier integrity. With the Colon Intestine-Chip, researchers can investigate novel mechanisms driving leaky gut syndrome and enable their translation from bench to patient bedside.

In this webinar, experts from Emulate and The Johns Hopkins University School of Medicine will share their studies and clear data on how the Colon Intestine-Chip can detect GI-related diseases.

Key points you will learn: 

  • The goal of the Johns Hopkins study of enteroids and how using the Emulate Colon Intestine-Chip taught them that flow, stretch, and physical forces make the enterocytes have greater resistance.

  • How physical forces revealed new aspects of host-pathogen effects.

  • Why data from Emulate suggests a novel mechanism of action of interleukin 22 (IL-22) on mature Intestinal Epithelial Cells (IECs).

  • How further high-throughput analysis in the Colon Intestine-Chip revealed that stretch enhances the functional maturation of the epithelium.

  • How Emulate leveraged interferon gamma (IFNγ), a cytokine hallmark of the progression of intestinal inflammation, to establish a reproducible model of cytokine-mediated barrier disruption.