Utilizing the Colon Intestine-Chip to Investigate Mechanisms of Gastrointestinal Disease

Organoids provide long-term viability, spatial organization, and cellular diversity representative of human intestinal epithelium. However, they don’t accurately mimic the architecture and the molecular signature of the intestinal epithelium. This limitation has been an impediment to allowing them to be harnessed for investigating mechanisms that drive leaky-gut syndrome in humans.

Emulate combines advancements in organoid and Organs-on-a-Chip technologies to develop a microphysiological Organ-on-Chip model designed to mimic properties of human intestinal epithelium to enable insights into barrier integrity. With the Colon Intestine-Chip, researchers can investigate novel mechanisms driving leaky gut syndrome and enable their translation from bench to patient bedside.

In this webinar, experts from Emulate and The Johns Hopkins University School of Medicine will share data from studies highlighting how the Colon Intestine-Chip can be used to investigate GI-related diseases.

Key points you will learn: 

  • How the enhanced microenvironment of Organ-Chips—including flow, stretch, and endothelial co-culture—promotes cell functionality, in vivo-like gene expression, and increased enterocyte resistance  
  • Novel insights into the effect of interleukin-22 (IL-22) on barrier function 
  • How the inclusion of mechanical forces brought new aspects of host-pathogen effects to light  
  • How Emulate created a reproducible cytokine-mediated barrier disruption model using interferon gamma (IFNγ)