Protocol to generate a 3D atherogenesis-on-chip model for studying endothelial-macrophage crosstalk in atherogenesis

Organ Model: Blood vessel (atherogenesis)

Application: Immunology & Inflammation

Highlights

  • Steps to develop an Organ-Chip model to study endothelial-macrophage interactions
  • Guidance for optimizing immunofluorescence microscopy in Organ-Chip platforms
  • Panel design for macrophage phenotyping using flow cytometry
  • Strategies for putative receptor-ligand analysis with qPCR and secretome analysis

Products Used In This Publication

Package design for the Chip-S1 Basic Research Kit
Chip-S1 Basic Research Kit
Render of the Emulate Human Emulation System on a white background, with the Zoë Culture Module in the front and the Orb Hub Module behind.
Zoë-CM2 Culture Module
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