Organ Model: Small Intestine (Jejunum)
Application: ADME
Highlights
Organ-Chips were used in this study to create a porcine jejunum model by seeding pig-derived intestinal organoids and human intestinal microvascular endothelial cells into a microfluidic chip with flow and peristalsis-like mechanical strain. The chips developed villi-like structures, crypt-like proliferative zones, and a differentiated epithelium containing enterocytes, goblet cells, and enteroendocrine cells. The system maintained a tight barrier, showed functional drug transporters (P-gp, BCRP, PEPT1) and inducible CYP3A metabolic activity, and responded to the permeability enhancer sodium caprate by reversibly opening and resealing the barrier—demonstrating its value as a predictive preclinical platform for studying intestinal permeability, transport, metabolism, and drug safety.
