Organ Model: Lung
Applications: Immunology & Inflammation
Highlights
This study developed a more realistic in vitro model of lung transplant cold storage ischemia/reperfusion injury (CS-IRI) using a lung alveolus-on-a-chip system. Human primary alveolar epithelial cells and human lung microvascular endothelial cells were cultured under 3D microfluidic air-liquid interface conditions with physiological flow and stretch. Chips were perfused with Perfadex and stored at 4°C for 6 h, then reperfused for 24 h at 37°C to simulate CS-IRI. Compared with control chips, CS-IRI caused loss of barrier function, increased inflammatory chemokines and adhesion molecules, and significant changes in 42 epithelial and 49 endothelial cell genes (P < 0.01). This model may improve understanding of CS-IRI after lung transplantation.
