GUIDED model
Lymphoid Organ-Chip
Recapitulate human vaccine and adjuvant responses
Model Overview
In vivo-like lymph node functionality
The Lymphoid Organ-Chip model supports the formation of lymphoid follicles with functional germinal centers similar to those found in secondary and tertiary lymphoid organs in vivo, enabling researchers to recapitulate human vaccine and adjuvant responses.
Benefits
3D Follicle Formation
Dynamic flow on-chip stimulates the assembly of large 3D multicellular aggregates.
Antigen-Specific Antibody Generation
Lymphoid Organ-Chips demonstrate significant anti-HA antibody generation following treatment with inactivated H5N1 virus.
Clinically Relevant Cytokine Production
The Lymphoid Organ-Chip displays clinically relevant levels of cytokines that are important for T cell expansion, survival, and helper functions.
Captures Donor-to-Donor Variability
Eight different human donors all produced anti-HA antibodies at detectable levels, with subsets of high and low responders identified.
Assess Responses to mRNA Vaccines
Myeloid cells capture and express mRNA vectored by lipid nanoparticles, enabling assessment of mRNA vaccines.
Neutralizing Antibody Production
Neutralizing antibody production against the SARS-CoV-2 spike protein has shown to be achieved after a culture duration of just six days.
The Lymphoid Organ-Chip is a Guided Model that Emulate has firsthand experience developing and supporting. It can be created using a Basic Research Kit and a user’s own cell sources. For Guided Models, Emulate offers guidelines and field scientist support to build a Lymphoid Organ-Chip using the Human Emulation System.
Case Studies
Community Publication
Ectopic Lymphoid Follicle Formation and Human Seasonal Influenza Vaccination Responses Recapitulated in an Organ-on-a-Chip
Read this publication to learn how researchers at the Wyss Institute developed a human model of ectopic lymphoid follicle formation, offering a new tool for studying lymphoid follicle development and a promising alternative to non-human primates for preclinical vaccine evaluation.
Community Publication
Modeling memory B cell responses in a lymphoid organ-chip to evaluate mRNA vaccine boosting
In this publication, researchers from the Institut Pasteur demonstrate how the Lymphoid Organ-Chip model can be used for the preclinical evaluation of vaccine-boosting strategies.
3d Follicle formation
Antigen & Adjuvant Response
Clinically Relevant Cytokine Production
Neutralizing Antibody Production
Featured Resource
Webinar
Development of a Lymphoid Organ-Chip to Evaluate mRNA Vaccine-Boosting Strategies
In this on-demand webinar, Lisa Chakrabarti, PhD, from Institut Pasteur described how her team developed a Lymphoid Organ-Chip using the Emulate Chip-S1® Stretchable Chip to address the challenge of predicting vaccine immunogenicity in humans.
Part of the Human Emulation System®
The Human Emulation System is comprised of instruments, consumables, and software in a flexible, open format. The user-friendly platform gives researchers a window into the inner workings of human biology.
