Organ Model: Mouth
Applications: Cancer
Highlights
This study presents a preliminary organ-on-a-chip model of oral dysplasia (OD-OoC) designed to better replicate the complex tissue interactions of precancerous lesions in the oral cavity. The microfluidic system integrates endothelial cells, gingival fibroblasts, and dysplastic oral keratinocytes across a collagen I–coated membrane, enabling organized three-dimensional cell–cell interactions under flow conditions. Imaging confirmed the presence and spatial organization of all cell types, as well as fibroblast migration across the membrane and dysplastic epithelial phenotypes distinct from healthy cells. This platform may provide a physiologically relevant in vitro tool to study epithelial transformation, cell migration, and early mechanisms involved in oral cancer development.
