Introduction
This protocol uses the Colon Intestine-Chip as a reference point aiming to the quantification of the percentage of actively proliferating epithelial cells, based on the incorporation and subsequent detection of 5-Ethynyl-2’-Deoxyuridine (EdU) in the newly synthesized DNA. The duration of the EdU pulse may differ with a different cell type or Organ-Chip. For the Colon Intestine-Chip it is recommended overnight pulse.