Introduction
This protocol uses the Primary Colon Intestine-Chip as a reference point aiming to the semi-quantitative assessment of the phosphorylated Signal transducer and activator of transcription 3 (STAT3) levels under baseline conditions and upon basolateral stimulation with the cytokine interleukin 22 (IL-22). These methods and assay conditions could change with a different Organ-Chip or stimulant.