Introduction
This protocol instructs users how to visualize the multifunctional scavenger receptor Stabilin-2 in Emulate Organ-Chips via immunofluorescence.
Stabilin-2 is a large, transmembrane receptor protein and is primarily expressed on sinusoidal endothelial cells of liver, spleen, and lymph node. The receptor has been shown to endocytose ligands such as low density lipoprotein, Gram-positive and Gram-negative bacteria, and advanced glycosylation end products.