Advanced Modeling of Inflammatory Immune Cell Recruitment and Response on Human Colon Intestine-Chip for IBD Therapeutic Development

Abstract

Immune cell recruitment into tissues is an essential step in inflammatory responses. This occurs in a highly tissue- and stimulus-specific manner, which presents a significant challenge to modeling disease and testing therapeutics ex vivo. We previously developed an advanced primary human vascularized Colon Intestine-Chip model and showed that it recapitulates physiologic cell composition, morphology and barrier integrity. The goal of this work was to test the ability of this system to model inflammatory bowel disease (IBD)-like immune cell responses.

The described model is advantageous in recapitulating in vivo inflammatory effector functions in that it supports immune cell trafficking under fluid flow conditions, uses primary cell co-culture, and provides a physiologically relevant peristaltic-like stretch.