In the effort to increase efficacy and specificity on newly developed drugs for intestinal disorders, human-based systems provide a promising new alternative to the animal-based studies. The interface of epithelium with endothelium allows for nutrient exchange and oxygen diffusion, as well as a gateway for circulating immune cells in states of inflammation. Our knowledge of the contribution of the microvasculature during intestinal mucosa development is still limited. We use Organ-Chips and our proprietary Human Emulation System to develop a platform where organoids are able to expand and differentiate to monolayers that consist of the three major intestinal epithelial cell types. Here, we report the development of a colonoid-based platform for disease modeling, biomarkers identification, and drug testing on a patient-specific approach.