Tagged: Lung-Chip

A Primary Human Lung Alveolus-on-a-Chip Model of Intravascular Thrombosis For Assessment of Therapeutics

Published in: Clinical Pharmacology & Therapeutics Pulmonary thrombosis is a significant cause of patient mortality, however, there are no effective in vitro models of thrombi formation in human lung microvessels, that could also assess therapeutics and toxicology of antithrombotic drugs. Here we show that a microfluidic lung alveolus-on-a-chip lined by human primary alveolar epithelium interfaced with endothelium,… Read more »

Matched-Comparative Modeling of Normal and Diseased Human Airway Responses Using a Microengineered Breathing Lung Chip

Published in: Cell Systems Smoking represents a major risk factor for chronic obstructive pulmonary disease (COPD), but it is diffi- cult to characterize smoke-induced injury responses under physiological breathing conditions in hu- mans due to patient-to-patient variability. Here, we show that a small airway-on-a-chip device lined by living human bronchiolar epithelium from normal or COPD… Read more »

Small Airway-on-a-Chip Enables Analysis of Human Lung Inflammation and Drug Responses In Vitro

Published in: Nature Methods Here we describe the development of a human lung ‘small airway-on-a-chip’ containing a differentiated, mucociliary bronchiolar epithelium and an underlying microvascular endothelium that experiences fluid flow, which allows for analysis of organ-level lung pathophysiology in vitro. Exposure of the epithelium to interleukin-13 (IL-13) reconstituted the goblet cell hyperplasia, cytokine hypersecretion and decreased… Read more »

Reconstituting Organ-Level Lung Functions on a Chip

Published in: Science Vol. 328 Here, we describe a biomimetic microsystem that reconstitutes the critical functional alveolar- capillary interface of the human lung. This bioinspired microdevice reproduces complex integrated organ-level responses to bacteria and inflammatory cytokines introduced into the alveolar space. In nanotoxicology studies, this lung mimic revealed that cyclic mechanical strain accentuates toxic and… Read more »

A Human Disease Model of Drug Toxicity–Induced Pulmonary Edema in a Lung-on-a-Chip Microdevice

Published in: Science Translational Medicine Vol. 4 Issue 159 Preclinical drug development studies currently rely on costly and time-consuming animal testing because existing cell culture models fail to recapitulate complex, organ-level disease processes in humans. We provide the proof of principle for using a biomimetic microdevice that reconstitutes organ-level lung functions to create a human… Read more »