Published in: Cell Death & Disease Studies on human intestinal injury induced by acute exposure to γ-radiation commonly rely on use of animal models because culture systems do not faithfully mimic human intestinal physiology. Here we used a human Gut-on-a-Chip (Gut Chip) microfluidic device lined by human intestinal epithelial cells and vascular endothelial cells to model radiation injury… Read more »
Published in: Cellular & Molecular Gastroenterology & Hepatology The 3-dimensional structure of human intestinal organoids makes them challenging to use. Here we describe how organoids, derived from induced pluripotent stem cells, can be incorporated into small microengineered Chips making them more amenable for study.
Published in: Scientific Reports Here we describe a method for fabricating a primary human Small Intestine-on-a-Chip (Intestine Chip) containing epithelial cells isolated from healthy regions of intestinal biopsies. The primary epithelial cells are expanded as 3D organoids, dissociated, and cultured on a porous membrane within a microfluidic device with human intestinal microvascular endothelium cultured in… Read more »
Published in: PLOS One Analysis of enterovirus infection is difficult in animals because they express different virus receptors than humans, and static cell culture systems do not reproduce the physical com- plexity of the human intestinal epithelium. Here, using coxsackievirus B1 (CVB1) as a proto- type enterovirus strain, we demonstrate that human enterovirus infection, replication… Read more »
Published in: Lab on a Chip March 5, 2012 Development of an in vitro living cell-based model of the intestine that mimics the mechanical, structural, absorptive, transport and pathophysiological properties of the human gut along with its crucial microbial symbionts could accelerate pharmaceutical development, and potentially replace animal testing. Here, we describe a biomimetic ‘human gut-on-a-chip’ microdevice… Read more »