Tagged: Organ-on-a-Chip

Introducing an Automated High Content Confocal Imaging Approach for Organs-on-Chips

Published in: Lab on a Chip Organ-Chips are micro-engineered systems that aim to recapitulate the organ microenvironment. Implementation of Organ-Chips within the pharmaceutical industry aims to improve the probability of success of drugs reaching late stage clinical trial by generating models for drug discovery that are of human origin and have disease relevance. We are… Read more »

Human iPSC-Derived Endothelial Cells and Microengineered Organ-Chip Enhance Neuronal Development

Published in: Stem Cell Reports Human stemcell-derived models of development and neurodegenerative diseases are challenged by cellular immaturity in vitro. Microengineered organ-on-chip (or Organ-Chip) systems are designed to emulate microvolume cytoarchitecture and enable co-culture of distinct cell types. Brainmicrovascular endothelial cells (BMECs) share commonsignaling pathwayswithneurons early indevelopment, but their contribution to human neuronalmaturation is largely unknown. To study… Read more »

Application of Microphysiological Systems to Enhance Safety Assessment in Drug Discovery

Published in: Annual Review of Pharmacology and Toxicology Enhancing the early detection of new therapies that are likely to carry a safety liability in the context of the intended patient population would provide a major advance in drug discovery. Microphysiological systems (MPS) technology offers an opportunity to support enhanced preclinical to clinical translation through the generation of higher-quality… Read more »

Organs-on-chips with integrated electrodes for trans-epithelial electrical resistance (TEER) measurements of human epithelial barrier function

Published in: Lab on a Chip Trans-epithelial electrical resistance (TEER) is broadly used as an experimental readout and a quality control assay for measuring the integrity of epithelial monolayers cultured under static conditions in vitro, however, there is no standard methodology for its application to microfluidic organ-on-a-chip (organ chip) cultures. Here, we describe a new microfluidic… Read more »

Cellular Mechanotransduction: Putting All The Pieces Together Again

Published in: The FASEB Journal Vol. 20 Analysis of cellular mechanotransduction, the mechanism by which cells convert mechanical signals into biochemical responses, has focused on identification of critical mechanosensitive molecules and cellular components. Stretch-activated ion channels, caveolae, integrins, cadherins, growth factor receptors, myosin motors, cytoskeletal filaments, nuclei, extracellular matrix, and numerous other structures and signaling… Read more »

Mechanobiology and Developmental Control

Published in: Annual Review of Cell & Development Biology 2013 Edition Morphogenesis is the remarkable process by which cells self-assemble into complex tissues and organs that exhibit specialized form and function during embryological development. Many of the genes and chemical cues that mediate tissue and organ formation have been identified; however, these signals alone are… Read more »

Microfabrication of Human Organs-On-Chips

Published in: Nature Protocols Vol. 8 No. 11 ‘Organs-on-chips’ are microengineered biomimetic systems containing microfluidic channels lined by living human cells, which replicate key functional units of living organs to reconstitute integrated human organ-level pathophysiology in vitro. These microdevices can be used to test efficacy and toxicity of drugs and chemicals, and to create in vitro models of… Read more »