Brain-Chip Characterization

Overview

Despite the growing prevalence of neurodegenerative disease globally, effective disease-modifying therapies remain largely out of reach, partially due to a lack of robust preclinical models that can be applied to model human brain physiology and predict blood brain barrier (BBB) penetration and efficacy of novel therapeutics.

In this note, we present data characterizing the Emulate Brain-Chip as a comprehensive model of the neurovascular unit (NVU), including a brain compartment recapitulating morphological and functional characteristics of human brain tissue and a functional BBB with low permeability comparable to in vivo. Compared to traditional cell culture models, the transcriptomic profile of the Brain-Chip more closely resembles mature adult human cortical tissue and maintains expression stability for several days. Taken together, the Brain-Chip is a physiologically relevant preclinical model of the human NVU that can improve investigation of brain physiology, neurodegenerative disease, drug efficacy, and drug transport across the BBB.

Key Highlights

  • Human-based model of the neurovascular unit—including neuronal compartment and BBB.
  • Tight BBB is formed with in vivo-like permeability that is improved by microglia.
  • Exhibits high transcriptomic similarity to adult human cortical tissue.
  • Enables neuroinflammatory disease modeling, target validation, and BBB penetration studies.