Triglycerides Quantification Assay



April 11, 2019

EP133 v.1.0

Goals:

Key Steps:

Other Required Materials:

Quantify triglycerides from Organ-Chip effluent

  • Prepare all reagents, samples, and standards
  • Triglyceride Assay Kit (Abcam ab65336)
  • Plate reader

Method

Sample type

Effluent

See Protocol EP124 Effluent Sampling.

Recommended effluent dilution (Liver-Chip)

1:5 (at flow rate 30 µL / h)

Run assay as described on vendor site

https://www.abcam.com/triglyceride-assay-kit-quantification-ab65336.html

Use vendor fluorometric protocol for best results.

Recommended sample quantification calculation

TGNet = TGeffluent TGtreatment media

TGNet = net effluent triglycerides (µg / mL)

TGeffluent = triglycerides from effluent (µg / mL)

TGtreatment media = triglycerides from treatment media (µg / mL)


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