March 28, 2019
Other Required Materials:
Detect and measure secreted cytokines and chemokines from Emulate Organ-Chip effluent samples
See Protocol EP124 Effluent Sampling.
Recommended assay flow rate (Liver-Chip)
30 µL / h
U-PLEX assay design
Assay plates can be customized by the user based on endpoint of interest.
Measured cytokines in the Liver-Chip typically include:
TNF-alpha, IL-1beta, IL-6, IL-8, IP-10, and MCP-1.
Liver-Chip effluent dilution
No dilution: samples are loaded neat.
Note: the dilution may be different if using different cell types.
Run assay as described on supplier site
Note: The U-PLEX Biomarker Group 1 (hu) contains 74 cytokines and chemokines. Up to 10 assays from this assay group may be multiplexed on each plate for simultaneous quantification.
Sample concentration range (Liver-Chip)
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The technology disclosed in this document may be covered by one or more patents or patent applications owned by or licensed to Emulate, Inc. No license is granted herein. Further information is available by contacting Emulate.