Multiplex Cytokine Assay



March 28, 2019

EP141 v1.0

Goals:

Key Steps:

Other Required Materials:

Detect and measure secreted cytokines and chemokines from Emulate Organ-Chip effluent samples

  • Prepare all reagents, samples, and standards
  • Run the assay
  • Read the plate
  • U-PLEX® Biomarker Group 1 Human Assays (MSD® Cat No. K15067L)
  • MSD instrument (electrochemiluminescence detection technology)

Method

Sample type

Organ-Chip effluent

 

See Protocol EP124 Effluent Sampling.

Recommended assay flow rate (Liver-Chip)

30 µL / h

U-PLEX assay design

Assay plates can be customized by the user based on endpoint of interest.

Measured cytokines in the Liver-Chip typically include:

TNF-alpha, IL-1beta, IL-6, IL-8, IP-10, and MCP-1.

Liver-Chip effluent dilution

(recommended)

No dilution: samples are loaded neat.

Note: the dilution may be different if using different cell types.

Run assay as described on supplier site

https://www.mesoscale.com/en/products/u-plex-biomarker-group-1-human-assays-k15067l/

Note: The U-PLEX Biomarker Group 1 (hu) contains 74 cytokines and chemokines. Up to 10 assays from this assay group may be multiplexed on each plate for simultaneous quantification.

Sample concentration range (Liver-Chip)

  • TNF-α ~ 2-4 pg / mL
  • ​IP-10 ~ 1- 60 pg / mL 
  • MCP-1 ~ 2–1,500 pg / mL 
  • IL-6 ~ 0.5-130 pg / mL

 


 

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Zoe™, Zoe-CM1™, Pod-1™ and Chip-S1™ are trademarks of Emulate, Inc.

MSD® and U-PLEX® are trademarks and/or service marks of Meso Scale Diagnostics, LLC

The technology disclosed in this document may be covered by one or more patents or patent applications owned by or licensed to Emulate, Inc. No license is granted herein. Further information is available by contacting Emulate.