April 4, 2019
Other Required Materials:
Visualize reactive oxygen species (ROS) via fluorescent imaging in live Organ-Chip
CellROX® is a fluorogenic probe for detecting cellular oxidative stress in live cells.
See Protocol EP155 Live Staining.
Recommended reagent dilution and incubation time
Dilute 1:500 in cell culture media. Incubate for 30 minutes at 37C.
Chips can be fixed immediately after staining: 4% PFA for 15 minutes at room temperature.
The signal remains for up to 2-hours after fixation for reagent C10422, and up to 3-days for reagent C10444.
Note: Do not fix cells before CellROX® staining.
See Protocol EP137 Fixation and Immunofluorescence (IF) Staining.
Image of the reactive oxygen species stained by CellROX® Deep Red reagent (cyan) in human hepatocytes treated with a compound in the Liver-Chip.
More information on vendor site
Note: We recommend using the aforementioned reagents for each organ model to achieve best results.
© Emulate, Inc., 2019. All rights reserved.
Zoe™, Zoe-CM1™, Pod-1™ and Chip-S1™ are trademarks of Emulate, Inc.
CellROX® is a trademark of Life Technologies, Inc.
The technology disclosed in this document may be covered by one or more patents or patent applications owned by or licensed to Emulate, Inc. No license is granted herein. Further information is available by contacting Emulate.