See Protocol EP155 Live Staining.
Recommended reagent dilution and incubation time
Prepare 1 mM stock solution in DMSO.
Dilute stock 1:5000 in serum-free media. Incubate for 30 minutes at 37C.
Chips can be fixed immediately after staining: 4% PFA for 15 minutes at room temperature.
The signal remains for up to 24 hours after fixation.
Note: Do not fix cells before MitoTracker® staining.
See Emulate Fixation and Immunofluorescence (IF) Staining for Organ-Chips (#6137).
Image of mitochondria (red) and nuclei (blue) in human hepatocytes in the Liver-Chip, stained by the MitoTracker® reagent (top channel).
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Organs-Chips in which the staining has been validated
MitoTracker® (Thermo Fisher Scientific TM M7510) has been validated on Liver-Chips.
MitoTracker® (Thermo Fisher Scientific TM M7513 and M22426) have been validated on Caco-2 Intestine-Chips.
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MitoTracker® is a registered trademark of Thermo Fisher Scientific.
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