April 7, 2019
Other Required Materials:
Visualize lipid droplets in Organ-Chips
Nile red is used to localize and quantify lipids, particularly neutral lipid droplets within cells.
See Protocol EP155 Live Staining of Cells.
Recommended reagent dilution and incubation time
Nile Red (Invitrogen™ N1142)
Prepare 30 mM stock in DMSO. Dilute 1:3000 in cell culture medium. Incubate for 30 minutes at room temperature.
Chips can be fixed immediately after staining: 4% PFA for 15 minutes at room temperature.
See Protocol EP137 Fixation and Immunofluorescence (IF) Staining.
Image of Nile red staining showing lipid droplets (red) / nuclei staining (blue) indicating presence of lipid droplets in hepatocytes in the human Liver-Chip (top channel)
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