Live Staining of Lipid Droplets Using AdipoRed



April 9, 2019

EP140 v1.0

 

Goals:

Key Steps:

Other Required Materials::

Visualization of intracellular lipid droplets in Emulate Organ-Chips

  • Live staining and fluorescent imaging in Organ-Chips
  • AdipoRedTM Adipogenesis Assay Reagent (Lonza, Cat # PT-7009)
  • PBS
  • Fluorescence microscope

Optional

  • 4% paraformaldehyde (PFA)

Background

AdipoRedTM is used to localize and quantify intracellular lipid droplets.

Method

Sample type

Live Organ-Chip

See Protocol EP155 Live Staining of Cells.

Recommended reagent dilution and incubation time

Dilute 1:40 in PBS. Incubate for 15 minutes at room temperature.

Note: Do not incubate for longer than 15 minutes as it will result in high background while imaging.

Fixative (optional)

Chips can be fixed immediately after staining: 4% PFA for 15 minutes at room temperature.

See Protocol EP137 Fixation and Immunofluorescence (IF) Staining.

Representative image

Image of AdipoRed (red) / nuclei staining (blue) indicating accumulation of lipids in hepatocytes in the human Liver-Chip (top channel).

More information on vendor site

https://bioscience.lonza.com/lonza_bs/US/en/Cell-analysis/p/000000000000186733/AdipoRed-Assay-Reagent%2C-20-mL

 


 

© Emulate, Inc., 2019. All rights reserved.

Emulate® and the Emulate logo are registered trademarks for Emulate, Inc.

AdipoRedTM is a trademark of Lonza Group or its subsidiaries.

The technology disclosed in this document may be covered by one or more patents or patent applications owned by or licensed to Emulate, Inc. No license is granted herein. You are solely responsible for determining whether you have all intellectual property rights that are necessary for your intended use of Emulate products or protocols, and whether you are required to obtain any additional intellectual property rights from a third party. Further information is available by contacting Emulate.