March 7, 2019
Other Required Materials:
Visualization of functional mitochondria using tetramethylrhodamine, methyl ester (TMRM) reagent via fluorescent imaging in live Organ-Chip
Tetramethylrhodamine, methyl ester (TMRM) is a cell-permeant dye that accumulates in healthy mitochondria with intact membrane potentials.
See Protocol EP155 Live Staining of Cells.
Recommended reagent dilution and incubation time
Dilute 1:1000 (final concentration 0.1 µM to 0.15 µM) in serum-free medium. Incubate for 30 minutes at 37C.
Image-iT™ TMRM reagent staining (red) of the active mitochondria and nuclei (blue) in human hepatocytes in the Liver-Chip.
More information on vendor site
Image-iT™ TMRM reagent has been validated in both Liver-Chips and Caco-2 Intestine-Chips.
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Image-iT™ is a trademark of Thermo Fisher Scientific or its subsidiaries
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