April 8, 2019
Other Required Materials:
Visualize activated caspase-3/7 via fluorescent imaging in live Organ-Chips
CellEvent™ Caspase-3/7 Green Detection Reagent is a fluorogenic probe for detecting activated caspase-3/7 in apoptotic cells.
See Protocol EP155 Live Staining.
Recommended reagent dilution and incubation time
CellEvent™ Caspase-3/7 Green Detection Reagent (InvitrogenTM C10423)
Dilute 1:250 – 1:1000 in PBS with 5% FBS. Incubate for 30 minutes at 37C.
Chips can be fixed immediately after staining: 4% PFA for 15 minutes at room temperature.
Note: Do not fix the chips before CellEvent™ Caspase-3/7 staining.
See Protocol EP137 Fixation and Immunofluorescence (IF) Staining.
Image of caspase-3/7 staining (green) indicating apoptotic hepatocytes in the Liver-Chip (top channel)
More information on vendor site
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