Live Staining of Caspase-3/7



April 8, 2019

EP158 v1.0

Goals:

Key Steps:

Other Required Materials:

Visualize activated caspase-3/7 via fluorescent imaging in live Organ-Chips

  • Live staining and fluorescent imaging in Organ-Chips
  • CellEvent™ Caspase-3/7 Green Detection Reagent (InvitrogenTM C10423)
  • PBS
  • Fetal bovine serum (FBS)
  • Fluorescence microscope

Optional

  • 4% paraformaldehyde (PFA)

Background

CellEvent™ Caspase-3/7 Green Detection Reagent is a fluorogenic probe for detecting activated caspase-3/7 in apoptotic cells.

Method

Sample type

Live Organ-Chip

See Protocol EP155 Live Staining.

Recommended reagent dilution and incubation time

CellEvent™ Caspase-3/7 Green Detection Reagent (InvitrogenTM C10423)

Dilute 1:250 – 1:1000 in PBS with 5% FBS. Incubate for 30 minutes at 37C.

Fixative (optional)

Chips can be fixed immediately after staining: 4% PFA for 15 minutes at room temperature.

Note: Do not fix the chips before CellEvent™ Caspase-3/7 staining.

See Protocol EP137 Fixation and Immunofluorescence (IF) Staining.

Representative image

Image of caspase-3/7 staining (green) indicating apoptotic hepatocytes in the Liver-Chip (top channel)

More information on vendor site

https://www.thermofisher.com/order/catalog/product/C10423


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InvitrogenTM and CellEvent™ are trademarks of Thermo Fisher Scientific or its subsidiaries.

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