LDH (Cytotoxicity) Assay



April 7, 2019

EP160 v1.0

Goals:

Key Steps:

Other Required Materials:

Quantify lactate dehydrogenase (LDH) levels from Emulate Organ-Chip effluent samples

  • Prepare all reagents, samples, and 100% cell lysate samples
  • CytoTox 96® Non-Radioactive Cytotoxicity Assay kit (Promega, G1780).
  • Plate reader capable of recording absorbance 490 or 492 nm

Method

Sample Type

Organ-Chip effluent

See Emulate Protocol EP124 Effluent Sampling.

Recommended assay flow rate (Liver-Chip)

30 µL / h

Recommended effluent dilution

No dilution: Samples are loaded neat

Notes:

  • Sample dilution may need to be adjusted further to accommodate for any experimental modifications by the user.
  • To measure maximum LDH release (100% cell lysate), follow manufacturer’s protocol. To perform cell lysis on a chip, see Emulate Protocol EP135 Cell Lysis for Protein Extraction.
  • Store effluent samples at room temperature prior to this assay.
  • Refrigeration or freezing of samples will deteriorate LDH enzyme and negatively affect assay data.

Run assay as described on supplier site

https://www.promega.com/Products/Cell-Health-Assays/Cell-Viability-and-Cytotoxicity-Assays/CytoTox-96-Non_Radioactive-Cytotoxicity-Assay/?fq=G1780&catNum=G1780

 


 

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CytoTox 96® is a registered trademarks of Promega Corporation.

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