April 23, 2019
Other Required Materials:
the multifunctional scavenger receptor stabilin-1 in Emulate Organ-Chips
This gene encodes a large, transmembrane receptor protein and is primarily expressed on sinusoidal endothelial cells of liver, spleen, and lymph node. The receptor has been shown to endocytose ligands such as low density lipoprotein, Gram-positive and Gram-negative bacteria, and advanced glycosylation end products.
See Emulate Protocol EP137 Fixation and Immunofluorescence (IF) Staining of Emulate Organ-Chips.
Recommended fixative and incubation time
4% PFA, 15 minutes at room temperature
Recommended permeabilization and incubation time
1% saponin in PBS, 30 minutes at room temperature
Recommended blocking buffer and incubation time
1% BSA, 10% goat serum in PBS, overnight at 4°C
Recommended primary antibody dilution and incubation time
Anti-Stabilin-1 (STAB1) (Novus NBP1-84444)
1:50 dilution in blocking buffer, overnight at 4°C
Recommended antibody host
Recommended secondary antibody dilution and incubation time
Alexa Fluor® 555-conjugated goat anti-rabbit IgG secondary antibody 1:500 dilution in blocking buffer, 2 hours at room temperature in the dark
Image of STAB-1 (red) / nuclei staining (blue) indicating presence of LSECs in the human Liver-Chip (bottom channel)
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