April 23, 2019
Other Required Materials:
Visualize expression of multidrug resistance-associated protein (MRP-2) in fixed Emulate Organ-Chips
See Emulate Protocol EP137 Fixation and Immunofluorescence (IF) Staining.
Fixative and incubation time (recommended)
4% PFA, 15 minutes at room temperature
Permeabilization and incubation time (recommended)
1% saponin in PBS, 30 minutes at room temperature
Blocking buffer and incubation time (recommended)
1% BSA, 10% goat serum in PBS, overnight at 4°C
Primary antibody dilution and incubation time (recommended)
Dilute anti-MRP-2 antibody [M2 III-6] (Abcam, ab3373)
1:100 in blocking buffer, overnight at 4°C.
Secondary antibody dilution and incubation time (recommended)
Dilute Alexa Fluor® 488-conjugated goat anti-mouse IgG secondary antibody 1:500 in blocking buffer for 2 hours at room temperature in the dark.
Image of MRP-2 (green) / nuclei (blue) staining indicating expression and polarization of MRP2 by hepatocytes in the human Liver-Chip (top channel)
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