Immunofluorescence Staining of F-actin

March 7, 2019

EP151 v1.0



Key Steps:

Other Required Materials:

Visualize F-actin via fluorescent imaging in Emulate Organ-Chips

  • Immunofluorescence staining in chip
  • Alexa Fluor™ 488 Phalloidin (InvitrogenTM A12379)
  • Dimethyl sulfoxide (DMSO)
  • PBS
  • 4% paraformaldehyde (PFA)
  • 10% Saponin
  • PBS
  • BSA
  • Fluorescence microscope


Sample type

Fixed Organ-Chip

See Protocol EP137 Fixation and Immunofluorescence (IF) Staining.

Recommended fixative and incubation time

4% PFA, 15 minutes at room temperature

Recommended permeabilization and incubation time

1% saponin in PBS, 30 minutes at room temperature

Recommended blocking buffer and incubation time

1% BSA,10% goat serum in PBS, overnight at 4°C

Recommended primary antibody dilution and incubation time

Alexa Fluor™ 488 Phalloidin (InvitrogenTM A12379)

Dilute 1:1000 in PBS (stock prepared in DMSO), 30 minutes incubation at room temperature

Representative image

Image of F-actin (green) in liver sinusoidal endothelial cells in the Liver-Chip (bottom channel).

More information on vendor site



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