March 7, 2019
Other Required Materials:
Visualize F-actin via fluorescent imaging in Emulate Organ-Chips
See Protocol EP137 Fixation and Immunofluorescence (IF) Staining.
Recommended fixative and incubation time
4% PFA, 15 minutes at room temperature
Recommended permeabilization and incubation time
1% saponin in PBS, 30 minutes at room temperature
Recommended blocking buffer and incubation time
1% BSA,10% goat serum in PBS, overnight at 4°C
Recommended primary antibody dilution and incubation time
Alexa Fluor™ 488 Phalloidin (InvitrogenTM A12379)
Dilute 1:1000 in PBS (stock prepared in DMSO), 30 minutes incubation at room temperature
Image of F-actin (green) in liver sinusoidal endothelial cells in the Liver-Chip (bottom channel).
More information on vendor site
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