April 8, 2019
Other Required Materials:
Visualize CD31 or platelet endothelial cell adhesion molecule (PECAM-1) in Organ- Chips
CD31 or PECAM-1, is a type I integral membrane glycoprotein that is expressed at high levels on endothelial cells. CD31 staining can be used as a marker of endothelial cells. The expression on endothelial cells is concentrated at junctions between adjacent cells.
See Protocol EP173 Fixation and Immunofluorescence (IF) Staining.
Recommended fixative and incubation time
4% PFA, 15 minutes at room temperature
Recommended permeabilization and incubation time
1% saponin in PBS, 30 minutes at room temperature
Recommended blocking buffer and incubation time
1% BSA, 10% goat serum in PBS, overnight at 4°C
Recommended primary antibody dilution and incubation time
Anti-CD31 (PECAM-1) (Abcam ab9498)
1:50 dilution in blocking buffer, overnight at 4°C
Recommended antibody host
Recommended secondary antibody dilution and incubation time
Alexa Fluor® 555-conjugated goat anti-mouse IgG secondary antibody 1:500 dilution in blocking buffer, 2 hours at room temperature in the dark.
Image of CD31 (red) / nuclei staining (blue) indicating presence of LSECs in the human Liver-Chip (bottom channel)
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