April 9, 2019
Other Required Materials:
Visualize bile salt export pump (BSEP) transporter in hepatocytes in fixed Liver-Chip
See Protocol EP137 Fixation and Immunofluorescence (IF) Staining.
Recommended fixative and incubation time
4% PFA, 15 minutes at room temperature
Recommended permeabilization and incubation time
1% saponin in PBS, 30 minutes at room temperature
Recommended blocking buffer and incubation time
1% BSA, 10% goat serum in PBS, overnight at 4°C
Recommended primary antibody dilution and incubation time
Anti-ABCB11 antibody (Abcam, ab155421)
1:100 dilution in blocking buffer, overnight at 4°C
Recommended antibody host
Recommended secondary antibody dilution and incubation time
Alexa Fluor™ 555-conjugated goat anti-rabbit IgG secondary antibody 1:500 in blocking buffer, 2 hours at room temperature in the dark
Image of BSEP transporter (red) / nuclei (blue) staining indicating localization of BSEP transporter in the bile canalicular membrane of hepatocytes in the human Liver-Chip (top channel).
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