April 4, 2019
Other Required Materials:
Visualize smooth-muscle actin filaments in fixed Emulate Organ-Chip
See Protocol EP137 Fixation and Immunofluorescence (IF) Staining.
Recommended fixative and incubation time
4% PFA, 15 minutes at room temperature
Recommended permeabilization and incubation time
1% saponin in PBS, 30 minutes at room temperature
Recommended blocking buffer and incubation time
1% BSA, 10% goat serum in PBS, overnight at 4°C
Recommended primary antibody dilution and incubation time
Anti-α-SMA antibody (Abcam, ab5694)
1:1000 dilution in blocking buffer, overnight at 4°C
Recommended antibody host
Recommended secondary antibody dilution and incubation time
Alexa Fluor™ 488-conjugated goat anti-rabbit IgG secondary antibody 1:500 dilution in blocking buffer, 2 hours at room temperature in the dark.
Image of α-SMA / nuclei staining indicating activated stellate cells in the human Liver-Chip (bottom channel)
More information on vendor site
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Zoe™, Zoe-CM1™, Pod-1™ and Chip-S1™ are trademarks of Emulate, Inc.
Alexa Fluor™ is a trademark of Life Technologies Corporation.
The technology disclosed in this document may be covered by one or more patents or patent applications owned by or licensed to Emulate, Inc. No license is granted herein. Further information is available by contacting Emulate.