Hepcidin Quantification Assay



EP134 v1.0

Goals:

Key Steps:

Other Required Materials:

Quantify hepcidin from Emulate Organ-Chip effluent

  • Prepare all reagents, samples, and standards
  • Run the assay
  • Read the plate
  • Human Hepcidin Quantikine® ELISA Kit (R&D Systems #DHP250)

Method

Sample type

Effluent

See Emulate Effluent Sampling Protocol (EP124)

Recommended effluent dilution using the Emulate Liver-Chip as example

1:2 to 1:200 (at flow rate 30 µL / h)

Note that hepcidin levels could change depending on cell injury status or based on donor-to-donor variability. Therefore, sample dilutions may need to be modified to accommodate different experimental conditions or cells from different donors.

Run assay as described on supplier site

https://www.rndsystems.com/products/human-hepcidin-quantikine-elisa-kit_dhp250

 


 

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Quantikine® is a registered trademark of R&D Systems, Inc.

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