Other Required Materials:
Quantify hepcidin from Emulate Organ-Chip effluent
See Emulate Effluent Sampling Protocol (EP124)
Recommended effluent dilution using the Emulate Liver-Chip as example
1:2 to 1:200 (at flow rate 30 µL / h)
Note that hepcidin levels could change depending on cell injury status or based on donor-to-donor variability. Therefore, sample dilutions may need to be modified to accommodate different experimental conditions or cells from different donors.
Run assay as described on supplier site
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