Hepcidin Quantification Assay

EP134 v1.0


Key Steps:

Other Required Materials:

Quantify hepcidin from Emulate Organ-Chip effluent

  • Prepare all reagents, samples, and standards
  • Run the assay
  • Read the plate
  • Human Hepcidin Quantikine® ELISA Kit (R&D Systems #DHP250)


Sample type


See Emulate Effluent Sampling Protocol (EP124)

Recommended effluent dilution using the Emulate Liver-Chip as example

1:2 to 1:200 (at flow rate 30 µL / h)

Note that hepcidin levels could change depending on cell injury status or based on donor-to-donor variability. Therefore, sample dilutions may need to be modified to accommodate different experimental conditions or cells from different donors.

Run assay as described on supplier site




© Emulate, Inc., 2019.  All rights reserved.

Emulate and the Emulate logo are registered trademarks of Emulate, Inc.

Quantikine® is a registered trademark of R&D Systems, Inc.

The technology disclosed in this document may be covered by one or more patents or patent applications owned by or licensed to Emulate, Inc.  No license is granted herein.  You are solely responsible for determining whether you have all intellectual property rights that are necessary for your intended use of Emulate products or protocols, and whether you are required to obtain any additional intellectual property rights from a third party. Further information is available by contacting Emulate.