Other Required Materials:
Quantify total glutathione (GSH) levels from Emulate Organ-Chip cell lysate samples
The GSH-Glo™ Assay is a luminescent-based assay for the detection and quantification of glutathione (GSH), an antioxidant that can prevent damage to cellular components caused by reactive oxygen species such as free radicals, peroxides, and lipid peroxides. GSH is involved in the detoxification of both xenobiotic and endogenous compounds. A change in GSH levels can be used as an indicator of toxicity.
Organ-Chip cell lysate
See Emulate Protocol EP135 Cell Lysis for Protein Analysis.
Recommended assay flow rate (Liver-Chip)
30 µL / h
Recommended cell lysate dilution (Liver-Chip)
No dilution: Samples are loaded neat.
Note: Sample dilution may need to be adjusted further to accommodate for any experimental modifications by the end-user.
Run assay as described on supplier site
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GSH-Glo™ is a trademark of the Promega Corporation.
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