GSH-Glo™ Glutathione Assay



EP159 v1.0

Goals:

Key Steps:

Other Required Materials:

Quantify total glutathione (GSH) levels from Emulate Organ-Chip cell lysate samples

  • Prepare all reagents, samples, and standards
  • Run the assay
  • Read plate
  • GSH-Glo™ Glutathione Assay (Promega #V6911)
  • Distilled or deionized water
  • PBS
  • White, opaque polystyrene flat-bottom 96- or 384-well plates
  • Luminescence plate reader

Introduction

The GSH-Glo™ Assay is a luminescent-based assay for the detection and quantification of glutathione (GSH), an antioxidant that can prevent damage to cellular components caused by reactive oxygen species such as free radicals, peroxides, and lipid peroxides. GSH is involved in the detoxification of both xenobiotic and endogenous compounds. A change in GSH levels can be used as an indicator of toxicity.

Method

Sample type

Organ-Chip cell lysate

See Emulate Protocol EP135 Cell Lysis for Protein Analysis.

Recommended assay flow rate (Liver-Chip)

30 µL / h

Recommended cell lysate dilution (Liver-Chip)

No dilution: Samples are loaded neat.

Note: Sample dilution may need to be adjusted further to accommodate for any experimental modifications by the end-user.

Run assay as described on supplier site

https://www.promega.com/products/cell-health-assays/oxidative-stress-assays/gsh_glo-glutathione-assay/?catNum=V6911

 


 

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Emulate® and the Emulate logo are registered trademarks for Emulate, Inc.

GSH-Glo™ is a trademark of the Promega Corporation.

The technology disclosed in this document may be covered by one or more patents or patent applications owned by or licensed to Emulate, Inc. No license is granted herein. You are solely responsible for determining whether you have all intellectual property rights that are necessary for your intended use of Emulate products or protocols, and whether you are required to obtain any additional intellectual property rights from a third party. Further information is available by contacting Emulate.