Other Required Materials:
Quantify alpha glutathione S-transferase (αGST) levels from Organ-Chip effluent samples
TECO® αGST is an ELISA for the quantitative determination of alpha Glutathione S-Transferase (αGST). GSTs are enzymes that catalyze the conjugation of glutathione to electrophilic centers on a wide variety of substrates in order to make the compounds more water-soluble. The role of GSTs is to detoxify endogenous compounds such as those that enable the breakdown of xenobiotics. High intracellular concentrations of GSTs coupled and released into serum are indicators of hepatocyte injury and toxicity and can be used as biomarkers. They are also used as indicators of renal injury and nephrotoxicity.
See Emulate Protocol EP124 Effluent Sampling Protocol.
Recommended assay flow rate (Liver-Chip)
30 µL / h
Recommended effluent dilution (Liver-Chip)
No dilution: Samples are loaded neat.
Note: Sample dilution may need to be adjusted further to accommodate for any experimental modifications by the end-user.
Run assay as described on supplier site
Note: Store kit at 2–8 °C immediately upon receipt. Do not freeze.
Expected sample concentration range (Liver-Chip)
1–40 ng / mL
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