April 4, 2019
Other Required Materials:
Quantify cholesterol from Organ-Chip effluent
For best results, use hepatocyte media without FBS and with ITSG (ThermoFisher #41400045) instead of the ITS premix that is used as normal Liver-Chip media.
See Protocol EP124 Effluent Sampling.
Recommended effluent dilution (Liver-Chip)
No dilution, or up to 1:10 (at flow rate 30 µL / h)
Note: Cholesterol concentration of media may change assay dilution range. Adjust accordingly.
Run assay as described on vendor site
Use vendor fluorometric protocol for best results.
Chol Net = Net effluent cholesterol (µg / mL)
Chol effluent = Cholesterol from effluent (µg / mL)
Chol dosing media = Cholesterol from dosing media (µg / mL)
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