Aspartate Transaminase (AST) Assay



 

April 8, 2019

EP147 v1.0

Goals:

Key Steps:

Required Materials:

Quantify human aspartate transaminase (AST) from Liver-Chip effluent samples

  • Prepare all reagents, samples, and standards
  • Run assay
  • Read plate
  • Human AST AlphaLISA® Detection Kit (Perkin Elmer AL3078HV)
  • EnVision® Alpha Reader

Method

Sample type

Liver-Chip effluent

See Protocol EP124 Effluent Sampling.

Recommended assay flow rate (Liver-Chip)

30 µL / h

Recommended effluent dilution (Liver-Chip)

No dilution: Samples are loaded neat.

Note: Sample dilution may need to be adjusted further to accommodate for any experimental modifications by the user.

Run assay as described on supplier site

http://www.perkinelmer.com/product/alphalisa-ast-human-kit-100pts-al3078hv

Note: Store kit immediately upon receipt in the dark at 4°C.

Sample concentration range (Liver-Chip)

500–20,000 pg / mL

 


 

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