Alanine Transaminase Activity Assay



April 1, 2019

EP143 v1.0

Goals:

Key Steps:

Other Required Materials:

Measurement of alanine transaminase (ALT) activity from Emulate Liver-Chip effluent samples

  • Prepare all reagents, samples, and standards
  • Run the assay
  • Read the plate
  • Alanine Transaminase Activity Assay Kit (Colorimetric / Fluorometric) (Abcam Cat No. ab105134)
  • Plate reader

Method

Sample type

Liver-Chip effluent

See Protocol EP124 Emulate Effluent Sampling.

Liver-Chip assay flow rate

(recommended)

30 µL / h

Liver-Chip effluent dilution

(recommended)

1:10

Note: Sample dilution may need to be adjusted further to accommodate for any experimental modifications by the user. ALT levels will change depending on cell injury status or based on donor-to-donor variability. Therefore, sample dilutions may need to be modified to accommodate different experimental conditions or cells from different donors.

Run assay as described on supplier site

https://www.abcam.com/alanine-transaminase-activity-assay-kit-colorimetricfluorometric-ab105134.html

Liver-Chip data range

150–3000 mU / mL

 


 

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