April 1, 2019
Other Required Materials:
Measurement of alanine transaminase (ALT) activity from Emulate Liver-Chip effluent samples
See Protocol EP124 Emulate Effluent Sampling.
Liver-Chip assay flow rate
30 µL / h
Liver-Chip effluent dilution
Note: Sample dilution may need to be adjusted further to accommodate for any experimental modifications by the user. ALT levels will change depending on cell injury status or based on donor-to-donor variability. Therefore, sample dilutions may need to be modified to accommodate different experimental conditions or cells from different donors.
Run assay as described on supplier site
Liver-Chip data range
150–3000 mU / mL
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