Topic: Publication

Introducing an Automated High Content Confocal Imaging Approach for Organs-on-Chips

Published in: Lab on a Chip Organ-Chips are micro-engineered systems that aim to recapitulate the organ microenvironment. Implementation of Organ-Chips within the pharmaceutical industry aims to improve the probability of success of drugs reaching late stage clinical trial by generating models for drug discovery that are of human origin and have disease relevance. We are… Read more »

Organ‐on‐Chip Recapitulates Thrombosis Induced by an anti‐CD154 Monoclonal Antibody: Translational Potential of Advanced Microengineered Systems

Published in: Clinical Pharmacology & Therapeutics Clinical development of Hu5c8, a monoclonal antibody against CD40L intended for treatment of autoimmune disorders, was terminated due to unexpected thrombotic complications. These life‐threatening side‐effects were not discovered during preclinical testing due to the lack of predictive models. In the present study, we describe the development of a microengineered… Read more »

Human iPSC-Derived Endothelial Cells and Microengineered Organ-Chip Enhance Neuronal Development

Published in: Stem Cell Reports Human stemcell-derived models of development and neurodegenerative diseases are challenged by cellular immaturity in vitro. Microengineered organ-on-chip (or Organ-Chip) systems are designed to emulate microvolume cytoarchitecture and enable co-culture of distinct cell types. Brainmicrovascular endothelial cells (BMECs) share commonsignaling pathwayswithneurons early indevelopment, but their contribution to human neuronalmaturation is largely unknown. To study… Read more »

Modeling radiation injury-induced cell death and countermeasure drug responses in a human Gut-on-a-Chip

Published in: Cell Death & Disease Studies on human intestinal injury induced by acute exposure to γ-radiation commonly rely on use of animal models because culture systems do not faithfully mimic human intestinal physiology. Here we used a human Gut-on-a-Chip (Gut Chip) microfluidic device lined by human intestinal epithelial cells and vascular endothelial cells to model radiation injury… Read more »

Enhanced Utilization of Induced Pluripotent Stem Cell–Derived Human Intestinal Organoids Using Microengineered Chips

Published in: Cellular & Molecular Gastroenterology & Hepatology The 3-dimensional structure of human intestinal organoids makes them challenging to use. Here we describe how organoids, derived from induced pluripotent stem cells, can be incorporated into small microengineered Chips making them more amenable for study.

Development of a primary human Small Intestine-on-a-Chip using biopsy-derived organoids

Published in: Scientific Reports Here we describe a method for fabricating a primary human Small Intestine-on-a-Chip (Intestine Chip) containing epithelial cells isolated from healthy regions of intestinal biopsies. The primary epithelial cells are expanded as 3D organoids, dissociated, and cultured on a porous membrane within a microfluidic device with human intestinal microvascular endothelium cultured in… Read more »

Application of Microphysiological Systems to Enhance Safety Assessment in Drug Discovery

Published in: Annual Review of Pharmacology and Toxicology Enhancing the early detection of new therapies that are likely to carry a safety liability in the context of the intended patient population would provide a major advance in drug discovery. Microphysiological systems (MPS) technology offers an opportunity to support enhanced preclinical to clinical translation through the generation of higher-quality… Read more »

Organs-on-chips with integrated electrodes for trans-epithelial electrical resistance (TEER) measurements of human epithelial barrier function

Published in: Lab on a Chip Trans-epithelial electrical resistance (TEER) is broadly used as an experimental readout and a quality control assay for measuring the integrity of epithelial monolayers cultured under static conditions in vitro, however, there is no standard methodology for its application to microfluidic organ-on-a-chip (organ chip) cultures. Here, we describe a new microfluidic… Read more »

A Primary Human Lung Alveolus-on-a-Chip Model of Intravascular Thrombosis For Assessment of Therapeutics

Published in: Clinical Pharmacology & Therapeutics Pulmonary thrombosis is a significant cause of patient mortality, however, there are no effective in vitro models of thrombi formation in human lung microvessels, that could also assess therapeutics and toxicology of antithrombotic drugs. Here we show that a microfluidic lung alveolus-on-a-chip lined by human primary alveolar epithelium interfaced with endothelium,… Read more »

Human Gut-On-A-Chip Supports Polarized Infection of Coxsackie B1 Virus In Vitro

Published in: PLOS One Analysis of enterovirus infection is difficult in animals because they express different virus receptors than humans, and static cell culture systems do not reproduce the physical com- plexity of the human intestinal epithelium. Here, using coxsackievirus B1 (CVB1) as a proto- type enterovirus strain, we demonstrate that human enterovirus infection, replication… Read more »